Hotspot

rpl32-trnL-UAG

SSC · 104717-105481 · 765 bp

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Pi 0.00504
MarkerSeek score 63.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
765
variable_sites
6
pi
0.00504
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGGTTTTTCTGGGCAACA TTCGAACCGAGATGCTTGAG 45.0 50.0 58.1 58.0 856-864 bp 1.0 80.3
rpl32-trnL-UAG_p2 AGGGTTTTTCTGGGCAACAA TTCGAACCGAGATGCTTGAG 45.0 50.0 58.1 58.0 855-863 bp 1.0 80.3
rpl32-trnL-UAG_p3 AAGGGTTTTTCTGGGCAACA GGATTCGAACCGAGATGCTT 45.0 50.0 58.1 58.1 859-867 bp 1.0 79.8
rpl32-trnL-UAG_p4 AGGGTTTTTCTGGGCAACAA GGATTCGAACCGAGATGCTT 45.0 50.0 58.1 58.1 858-866 bp 1.0 79.8
rpl32-trnL-UAG_p5 AGGGTTTTTCTGGGCAACAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 828-836 bp 1.0 79.3