Hotspot

rpl20-rps12

LSC · 66294-66988 · 695 bp

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Pi 0.00264
MarkerSeek score 51.10
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
695
variable_sites
3
pi
0.00264
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 ACGCATTTTTGTTCGTCGTC GAGGAACATGTGCTAGGGTG 45.0 55.0 58.0 58.0 772 bp 1.0 80.0
rpl20-rps12_p2 ACGCATTTTTGTTCGTCGTC GTCGAGGAACATGTGCTAGG 45.0 55.0 58.0 58.1 775 bp 1.0 79.6
rpl20-rps12_p3 ACGCATTTTTGTTCGTCGTC ATGCCGACGGTTAAACAACT 45.0 45.0 58.0 58.1 863 bp 1.0 79.3
rpl20-rps12_p4 GTTTGAGGCAAATGAACGCA GAGGAACATGTGCTAGGGTG 45.0 55.0 57.9 58.0 787 bp 1.0 79.0
rpl20-rps12_p5 GTTTGAGGCAAATGAACGCA GTCGAGGAACATGTGCTAGG 45.0 55.0 57.9 58.1 790 bp 1.0 78.7