Hotspot

rbcL-psaI

LSC · 55288-56908 · 1621 bp

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Pi 0.00257
MarkerSeek score 34.50
Primer available yes
Label rbcL-psaI

Feature Metadata

label_name
rbcL-psaI
feature_type
igs
region
LSC
length_bp
1621
variable_sites
8
pi
0.00257
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.67
markerseek_score
34.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-psaI_p1 AGTTCGAGCCGGTAGATACT GAAGGTAAGTTTAAATCCGTCA 50.0 36.4 57.7 54.2 1137-1678 bp 1.0 44.5
rbcL-psaI_p2 GGCGATCAAATTCGAGTTCG GAAGGTAAGTTTAAATCCGTCA 50.0 36.4 57.6 54.2 1151-1692 bp 1.0 44.4
rbcL-psaI_p3 TGGAAGGCGATCAAATTCGA GAAGGTAAGTTTAAATCCGTCA 45.0 36.4 57.6 54.2 1156-1697 bp 1.0 44.4
rbcL-psaI_p4 AGCTTGCAAATGGAGTCCTG GAAGGTAAGTTTAAATCCGTCA 50.0 36.4 58.5 54.2 1199-1740 bp 1.0 44.4
rbcL-psaI_p5 CAGCTTGCAAATGGAGTCCT GAAGGTAAGTTTAAATCCGTCA 50.0 36.4 58.5 54.2 1200-1741 bp 1.0 44.4