Hotspot

ndhC-trnV-UAC

LSC · 49255-50103 · 849 bp

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Pi 0.000792
MarkerSeek score 51.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
849
variable_sites
1
pi
0.000792
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GCCCTGAAATCCAAAATGCC GGTTCGAATCCGTATAGCCC 50.0 55.0 58.0 57.9 946-959 bp 1.0 80.3
ndhC-trnV-UAC_p2 GCCCTGAAATCCAAAATGCC CGGTTCGAATCCGTATAGCC 50.0 55.0 58.0 58.3 947-960 bp 1.0 78.5
ndhC-trnV-UAC_p3 CTCTGGTCCTTCACTAACCG GGTTCGAATCCGTATAGCCC 55.0 55.0 57.3 57.9 975-988 bp 1.0 75.3
ndhC-trnV-UAC_p4 TCTGGTCCTTCACTAACCGG GGTTCGAATCCGTATAGCCC 55.0 55.0 58.7 57.9 974-987 bp 1.0 74.6
ndhC-trnV-UAC_p5 TCACTAACCGGGGCTAAAAG GGTTCGAATCCGTATAGCCC 50.0 55.0 57.2 57.9 965-978 bp 1.0 74.4