Hotspot

petN-trnC-GCA

LSC · 16912-17779 · 868 bp

Back to Phleum

Pi 0.00423
MarkerSeek score 61.90
Primer available yes
Label petN-trnC-GCA

Feature Metadata

label_name
petN-trnC-GCA
feature_type
igs
region
LSC
length_bp
868
variable_sites
6
pi
0.00423
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-trnC-GCA_p1 AGCCCAAGCGAGACTTACTA TTTCTCGATTTTCGCTCGGA 50.0 45.0 58.2 57.6 988-992 bp 1.0 76.7
petN-trnC-GCA_p2 CCACTCCTCCCCCATACTAC TTTCTCGATTTTCGCTCGGA 60.0 45.0 58.3 57.6 1044-1048 bp 1.0 75.7
petN-trnC-GCA_p3 AGCCCAAGCGAGACTTACTA TCTCGATTTTCGCTCGGATT 50.0 45.0 58.2 57.4 986-990 bp 1.0 75.0
petN-trnC-GCA_p4 AGCCCAAGCGAGACTTACTA TTCTCGATTTTCGCTCGGAT 50.0 45.0 58.2 57.4 987-991 bp 1.0 75.0
petN-trnC-GCA_p5 TCCACTCCTCCCCCATACTA TTTCTCGATTTTCGCTCGGA 55.0 45.0 58.4 57.6 1045-1049 bp 1.0 74.9