Hotspot

psbK-psbI

LSC · 6891-7292 · 402 bp

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Pi 0.0029
MarkerSeek score 56.80
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
402
variable_sites
2
pi
0.0029
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG GAATTTTTACTCCTCGCGCC 50.0 50.0 57.9 57.5 548-551 bp 1.0 76.4
psbK-psbI_p2 TGGCAAGCTGCTGTAAGTTT GAATTTTTACTCCTCGCGCC 45.0 50.0 58.3 57.5 545-548 bp 1.0 74.3
psbK-psbI_p3 TTGGCAAGCTGCTGTAAGTT GAATTTTTACTCCTCGCGCC 45.0 50.0 58.3 57.5 546-549 bp 1.0 74.3
psbK-psbI_p4 TTTGGCAAGCTGCTGTAAGT GAATTTTTACTCCTCGCGCC 45.0 50.0 58.3 57.5 547-550 bp 1.0 74.3
psbK-psbI_p5 GTTTGGCAAGCTGCTGTAAG AATTTTTACTCCTCGCGCCC 50.0 50.0 57.9 58.9 547-550 bp 1.0 72.7