Hotspot

ndhA

Genome · 123450-125380 · 1931 bp

Back to Phlegmariurus

Pi 0.5

MarkerSeek score 79.30

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: Genome
length_bp: 1931
variable_sites: 801
pi: 0.5
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ATACTCGACACAGTTTCCGC	AAATTTAGAGGCCCGACGAC	50.0	50.0	58.0	58.0	2687 bp	0.5	59.8
ndhA_p2	ATACTCGACACAGTTTCCGC	GTCCTATGCTACGAGCTTCG	50.0	55.0	58.0	58.0	2889 bp	0.5	59.6
ndhA_p3	CTTCCACCACTGATAAGGGC	AAATTTAGAGGCCCGACGAC	55.0	50.0	58.0	58.0	2802 bp	0.5	59.5
ndhA_p4	ATACTCGACACAGTTTCCGC	ATTTAGAGGCCCGACGACTA	50.0	50.0	58.0	57.9	2685 bp	0.5	59.4
ndhA_p5	ATACTCGACACAGTTTCCGC	TAGAGGCCCGACGACTAAAT	50.0	50.0	58.0	57.9	2682 bp	0.5	59.4