Hotspot

rps15-ycf1

Genome · 126827-127365 · 539 bp

Back to Phlegmariurus

Pi 0.443

MarkerSeek score 80.50

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: Genome
length_bp: 539
variable_sites: 231
pi: 0.443
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TGTAGCAAGCACGGTCATAC	TGGGAGTCGATTTGCTATGC	50.0	50.0	58.0	58.0	875 bp	0.5	59.6
rps15-ycf1_p2	AACAGGTGAGAAGCTATGCG	TGGGAGTCGATTTGCTATGC	50.0	50.0	58.0	58.0	1204 bp	0.5	59.5
rps15-ycf1_p3	TGTAGCAAGCACGGTCATAC	ACCGCGAGTATTTGATGACC	50.0	50.0	58.0	58.1	1292 bp	0.5	59.5
rps15-ycf1_p4	TGTAGCAAGCACGGTCATAC	CTACTTCCAAAGCGCCGTAT	50.0	50.0	58.0	58.1	1111 bp	0.5	59.5
rps15-ycf1_p5	TGTAGCAAGCACGGTCATAC	CCGCGAGTATTTGATGACCA	50.0	50.0	58.0	58.1	1291 bp	0.5	59.4