Hotspot

trnA-UGC-rrn23

Genome · 107252-108267 · 1016 bp

Back to Phlegmariurus

Pi 0.0394
MarkerSeek score 83.00
Primer available yes
Label trnA-UGC-rrn23

Feature Metadata

label_name
trnA-UGC-rrn23
feature_type
igs
region
Genome
length_bp
1016
variable_sites
39
pi
0.0394
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnA-UGC-rrn23_p1 TCCCGGCCTTCTTGGTATAA GTCTCTGGGTGCCTAGGTAT 50.0 55.0 58.1 57.9 1155-1165 bp 1.0 82.9
trnA-UGC-rrn23_p2 TTCCCGGCCTTCTTGGTATA GTCTCTGGGTGCCTAGGTAT 50.0 55.0 58.1 57.9 1156-1166 bp 1.0 82.9
trnA-UGC-rrn23_p3 TCCCGGCCTTCTTGGTATAA GAATCTCCGGATCCATGCTC 50.0 55.0 58.1 57.9 1221-1231 bp 1.0 82.6
trnA-UGC-rrn23_p4 TTCCCGGCCTTCTTGGTATA GAATCTCCGGATCCATGCTC 50.0 55.0 58.1 57.9 1222-1232 bp 1.0 82.6
trnA-UGC-rrn23_p5 TCCCGGCCTTCTTGGTATAA TCATTTTCAGCTCCCCGAAG 50.0 50.0 58.1 57.9 1203-1213 bp 1.0 82.5