Hotspot

trnS-UGA-psbZ

LSC · 38157-38470 · 314 bp

Back to Phippsiomeles

Pi 0.0446

MarkerSeek score 78.40

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 314
variable_sites: 14
pi: 0.0446
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAAGACCGGAGCTATCAACC	CAACCCTCAGGAGAAGCAAA	55.0	50.0	58.1	57.7	448-450 bp	1.0	79.8
trnS-UGA-psbZ_p2	CAAGACCGGAGCTATCAACC	TTTTGTTACCCGACCAACCC	55.0	50.0	58.1	58.3	462-464 bp	1.0	79.5
trnS-UGA-psbZ_p3	CTATCAACCACTCGGCCATC	CAACCCTCAGGAGAAGCAAA	55.0	50.0	58.1	57.7	437-439 bp	1.0	79.3
trnS-UGA-psbZ_p4	CTATCAACCACTCGGCCATC	TTTTGTTACCCGACCAACCC	55.0	50.0	58.1	58.3	451-453 bp	1.0	79.0
trnS-UGA-psbZ_p5	AAGACCGGAGCTATCAACCA	CAACCCTCAGGAGAAGCAAA	50.0	50.0	58.4	57.7	447-449 bp	1.0	76.8