Hotspot

psbC-trnS-UGA

LSC · 37809-38063 · 255 bp

Back to Phippsiomeles

Pi 0

MarkerSeek score 33.80

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 255
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.4	58.1	405 bp	1.0	76.5
psbC-trnS-UGA_p2	TAGGTCATTTATGGCACGCG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.4	58.1	439 bp	1.0	76.2
psbC-trnS-UGA_p3	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.6	58.1	404 bp	1.0	76.0
psbC-trnS-UGA_p4	AGCTGCAGCAGGATTTGAAA	GATGGCCGAGTGGTTGATAG	45.0	55.0	58.4	58.1	416 bp	1.0	76.0
psbC-trnS-UGA_p5	TAGGTCATTTATGGCACGCG	GATGGCCGAGTGGTTGATAG	50.0	55.0	58.4	58.1	450 bp	1.0	75.6