Hotspot

rpoB-trnC-GCA

LSC · 27679-28895 · 1217 bp

Back to Phippsiomeles

Pi 0.00576

MarkerSeek score 62.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1217
variable_sites: 7
pi: 0.00576
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TAAGCCCTGCTCAATGAACC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.9	58.1	1372-1383 bp	1.0	78.8
rpoB-trnC-GCA_p2	TAAGCCCTGCTCAATGAACC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.9	58.4	1354-1365 bp	1.0	76.7
rpoB-trnC-GCA_p3	TATTGCCTCATTTCCACCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1306-1317 bp	1.0	76.3
rpoB-trnC-GCA_p4	CTCTGTTAAGCCCTGCTCAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1378-1389 bp	1.0	76.0
rpoB-trnC-GCA_p5	GTTCCTCTGTTAAGCCCTGC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.5	58.1	1382-1393 bp	1.0	75.5