Hotspot

trnH-GUG-psbA

LSC · 118-395 · 278 bp

Back to Phippsiomeles

Pi 0.0433
MarkerSeek score 69.30
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
278
variable_sites
12
pi
0.0433
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 ATATTATGGGCGAACGACGG CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 403 bp 1.0 81.5
trnH-GUG-psbA_p2 ATATTATGGGCGAACGACGG GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 453 bp 1.0 81.4
trnH-GUG-psbA_p3 ATATTATGGGCGAACGACGG TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 406 bp 1.0 81.4
trnH-GUG-psbA_p4 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 406 bp 1.0 81.2
trnH-GUG-psbA_p5 AATCCACTGCCTTGATCCAC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 405 bp 1.0 81.2