Hotspot

ndhA

SSC · 120954-123180 · 2227 bp

Back to Philotheca

Pi 0.0168

MarkerSeek score 55.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2227
variable_sites: 67
pi: 0.0168
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ATTCAATTCGTGACGGTCGT	TGTGAGAGTAGAAGCCCCAA	45.0	50.0	57.9	58.0	2921-2934 bp	1.0	81.9
ndhA_p2	GGCGAATTCAACACTGTTCG	ATGGGGGAAGTTGATCGTTG	50.0	50.0	58.0	57.9	2817-2830 bp	1.0	81.2
ndhA_p3	ACCAATGTACCTTGCAGCTC	TGTGAGAGTAGAAGCCCCAA	50.0	50.0	58.2	58.0	2582-2593 bp	0.75	70.0
ndhA_p4	GACCAATGTACCTTGCAGCT	TGTGAGAGTAGAAGCCCCAA	50.0	50.0	58.2	58.0	2583-2594 bp	0.75	70.0
ndhA_p5	GGCGAATTCAACACTGTTCG	TGTGAGAGTAGAAGCCCCAA	50.0	50.0	58.0	58.0	2998 bp	0.25	49.4