Hotspot

rpl32-trnL-UAG

SSC · 114602-115268 · 667 bp

Back to Philotheca

Pi 0.033

MarkerSeek score 69.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 667
variable_sites: 42
pi: 0.033
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCGTTGAAGGCTTTTTCGTT	TGCCTCGAGTAAATCCAACG	45.0	50.0	58.2	58.0	1105-1123 bp	1.0	83.9
rpl32-trnL-UAG_p2	CCAGAGCGTTCCCCAATAAA	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.9	58.0	1107-1127 bp	0.75	72.3
rpl32-trnL-UAG_p3	CCAGAGCGTTCCCCAATAAA	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.9	58.0	1108-1128 bp	0.75	72.3
rpl32-trnL-UAG_p4	CCAGAGCGTTCCCCAATAAA	TGCCTCGAGTAAATCCAACG	50.0	50.0	57.9	58.0	1458-1470 bp	0.75	72.0
rpl32-trnL-UAG_p5	CCAGAGCGTTCCCCAATAAA	GGATCCACATACAAGGGCTG	50.0	55.0	57.9	58.0	1726-1738 bp	0.75	71.4