Hotspot

trnS-GGA-rps4

LSC · 46602-46821 · 220 bp

Back to Philotheca

Pi 0.0462

MarkerSeek score 77.40

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 220
variable_sites: 20
pi: 0.0462
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	GACTTTTGTTTACCGGGGGT	AGTCAATGGGTCGGTTTGAA	50.0	45.0	58.0	57.4	325-404 bp	1.0	78.9
trnS-GGA-rps4_p2	AGACTTTTGTTTACCGGGGG	AGTCAATGGGTCGGTTTGAA	50.0	45.0	57.7	57.4	326-405 bp	1.0	76.7
trnS-GGA-rps4_p3	GACTTTTGTTTACCGGGGGT	AGTCAATGGGTCGGTTTGAAA	50.0	42.9	58.0	58.1	325-404 bp	1.0	75.6
trnS-GGA-rps4_p4	ATGTAGGAGAGATGGCCGAG	AGTCAATGGGTCGGTTTGAA	55.0	45.0	58.4	57.4	378-457 bp	1.0	75.5
trnS-GGA-rps4_p5	ACTTTTGTTTACCGGGGGTT	AGTCAATGGGTCGGTTTGAA	45.0	45.0	57.5	57.4	324-403 bp	1.0	75.3