Hotspot

psbZ-trnG-GCC

LSC · 37375-37905 · 531 bp

Back to Philotheca

Pi 0.037

MarkerSeek score 78.90

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 531
variable_sites: 36
pi: 0.037
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CGGATGGTTGGTCGAGTAAC	AATCGAACCCGCATCTTCTC	55.0	50.0	58.4	58.1	674-716 bp	1.0	83.5
psbZ-trnG-GCC_p2	CGGATGGTTGGTCGAGTAAC	GAATCGAACCCGCATCTTCT	55.0	50.0	58.4	58.1	675-717 bp	1.0	83.5
psbZ-trnG-GCC_p3	CGGATGGTTGGTCGAGTAAC	TTCCTTGACTCTGGCGGATA	55.0	50.0	58.4	58.1	699-741 bp	1.0	82.6
psbZ-trnG-GCC_p4	TTTGCTTCTCCGGATGGTTG	AATCGAACCCGCATCTTCTC	50.0	50.0	58.5	58.1	684-726 bp	1.0	82.5
psbZ-trnG-GCC_p5	TTTGCTTCTCCGGATGGTTG	GAATCGAACCCGCATCTTCT	50.0	50.0	58.5	58.1	685-727 bp	1.0	82.5