Hotspot

rpoB-trnC-GCA

LSC · 27098-28315 · 1218 bp

Back to Philotheca

Pi 0.0317

MarkerSeek score 72.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1218
variable_sites: 75
pi: 0.0317
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTCCCGCATTTCCATCTCC	ACGACTCGAATCTTTCCGTG	50.0	50.0	58.0	58.0	1468-1535 bp	1.0	87.7
rpoB-trnC-GCA_p2	ATTCCCGCATTTCCATCTCC	CACGACTCGAATCTTTCCGT	50.0	50.0	58.0	58.0	1469-1536 bp	1.0	87.7
rpoB-trnC-GCA_p3	CCGCCTACACAAGCAAATTG	ACGACTCGAATCTTTCCGTG	50.0	50.0	58.0	58.0	2146-2213 bp	1.0	85.3
rpoB-trnC-GCA_p4	CCGCCTACACAAGCAAATTG	CACGACTCGAATCTTTCCGT	50.0	50.0	58.0	58.0	2147-2214 bp	1.0	85.3
rpoB-trnC-GCA_p5	ATTCCCGCATTTCCATCTCC	GTTATACCAGCCAAGCCGAA	50.0	50.0	58.0	58.0	1367 bp	0.25	49.6