Hotspot

atpH-atpI

LSC · 13684-14796 · 1113 bp

Back to Philotheca

Pi 0.0274

MarkerSeek score 68.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1113
variable_sites: 57
pi: 0.0274
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CCGTTGCTTTAGCTTTGCTC	45.0	50.0	58.0	58.0	1480-1632 bp	1.0	86.8
atpH-atpI_p2	CCCTTTTCCCTTTGCTCCTT	CCGTTGCTTTAGCTTTGCTC	50.0	50.0	58.0	58.0	1991-2144 bp	1.0	86.5
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	TTCGGAATCCACAAACCGTT	45.0	45.0	58.0	58.0	1717-1869 bp	1.0	86.4
atpH-atpI_p4	CCCTTTTCCCTTTGCTCCTT	TTCGGAATCCACAAACCGTT	50.0	45.0	58.0	58.0	2228-2381 bp	1.0	86.3
atpH-atpI_p5	ACGTTGCTTTGTCTGGACTT	TTCGGAATCCACAAACCGTT	45.0	45.0	58.0	58.0	2395-2548 bp	0.75	71.8