Hotspot

trnR-UCU-atpA

LSC · 10240-10450 · 211 bp

Back to Philotheca

Pi 0.0498

MarkerSeek score 77.60

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 211
variable_sites: 19
pi: 0.0498
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TCAACTCGGCGATGCATTAA	CCAGTCAGGAGATGTTTCGG	45.0	55.0	58.0	58.0	978-1012 bp	1.0	85.6
trnR-UCU-atpA_p2	TAATGCATCGCCGAGTTGAA	CCAGTCAGGAGATGTTTCGG	45.0	55.0	58.0	58.0	959 bp	0.25	49.7
trnR-UCU-atpA_p3	TAATGCATCGCCGAGTTGAA	CCCAGTCAGGAGATGTTTCG	45.0	55.0	58.0	58.0	960 bp	0.25	49.7
trnR-UCU-atpA_p4	TTAATGCATCGCCGAGTTGA	CCAGTCAGGAGATGTTTCGG	45.0	55.0	58.0	58.0	960 bp	0.25	49.7
trnR-UCU-atpA_p5	TTAATGCATCGCCGAGTTGA	CCCAGTCAGGAGATGTTTCG	45.0	55.0	58.0	58.0	961 bp	0.25	49.7