Hotspot

trnS-GCU-trnG-UCC

LSC · 8246-9189 · 944 bp

Back to Philotheca

Pi 0.0291

MarkerSeek score 71.20

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 944
variable_sites: 52
pi: 0.0291
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CCGACGCTTTAATCCACTCA	AGGGGATTACTTGAACGAATCA	50.0	40.9	58.0	57.2	1014-1084 bp	1.0	63.6
trnS-GCU-trnG-UCC_p2	CCGACGCTTTAATCCACTCA	GGGGATTACTTGAACGAATCAC	50.0	45.5	58.0	57.1	1013-1083 bp	1.0	63.2
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	AGGGGATTACTTGAACGAATCA	50.0	40.9	58.2	57.2	1072-1142 bp	1.0	62.0
trnS-GCU-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	GGGGATTACTTGAACGAATCAC	50.0	45.5	58.2	57.1	1071-1141 bp	1.0	61.7
trnS-GCU-trnG-UCC_p5	ACGCTTTAATCCACTCAGCC	AGGGGATTACTTGAACGAATCA	50.0	40.9	58.3	57.2	1011-1081 bp	1.0	61.5