Hotspot

rps16-trnQ-UUG

LSC · 6143-6932 · 790 bp

Back to Philotheca

Pi 0.0295
MarkerSeek score 72.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
790
variable_sites
44
pi
0.0295
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CCACAACGGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 882-907 bp 1.0 82.8
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 861-886 bp 1.0 82.7
rps16-trnQ-UUG_p3 CCACAACGGATCATGTCCTT GAAATTGAAATGGGGCGTCG 50.0 50.0 57.9 58.1 940-965 bp 1.0 82.4
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GAAATTGAAATGGGGCGTCG 45.0 50.0 58.1 58.1 919-944 bp 1.0 82.3
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 872-897 bp 1.0 82.1