Hotspot

trnH-GUG-psbA

LSC · 77-443 · 367 bp

Back to Philotheca

Pi 0.046
MarkerSeek score 74.60
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
367
variable_sites
32
pi
0.046
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TGAAGCTCCATCTACAAATGG 55.0 42.9 58.1 55.7 411-429 bp 1.0 59.3
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA TGAAGCTCCATCTACAAATGG 50.0 42.9 57.9 55.7 425-443 bp 1.0 59.0
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC TGAAGCTCCATCTACAAATGG 50.0 42.9 57.9 55.7 424-442 bp 1.0 59.0
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC TGAAGCTCCATCTACAAATGG 50.0 42.9 57.9 55.7 426-444 bp 1.0 59.0
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG TTGAAGCTCCATCTACAAATGG 55.0 40.9 58.1 56.4 412-430 bp 1.0 57.3