Hotspot

ycf1

SSC · 119955-125627 · 5673 bp

Back to Philodendron

Pi 0.412

MarkerSeek score 73.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5673
variable_sites: 1856
pi: 0.412
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCGAGTGGTACGAGGATTTG	AATAGTCCTCGTGTCCCACA	55.0	50.0	58.1	58.1	1231 bp	0.5	58.8
ycf1_p2	CCGAGTGGTACGAGGATTTG	ATAGTCCTCGTGTCCCACAA	55.0	50.0	58.1	58.1	1230 bp	0.5	58.8
ycf1_p3	GAACTCCCTTTCGGTTCTCC	AATAGTCCTCGTGTCCCACA	55.0	50.0	57.9	58.1	971 bp	0.5	58.7
ycf1_p4	GAACTCCCTTTCGGTTCTCC	ATAGTCCTCGTGTCCCACAA	55.0	50.0	57.9	58.1	970 bp	0.5	58.7
ycf1_p5	GGAACTCCCTTTCGGTTCTC	ATAGTCCTCGTGTCCCACAA	55.0	50.0	57.9	58.1	971 bp	0.5	58.7