Hotspot

ndhH

SSC · 126805-127986 · 1182 bp

Back to Philodendron

Pi 0.492

MarkerSeek score 79.10

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 458
pi: 0.492
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GTGTGTAGTCTGTGAGCGAG	GCTTGGTACCATCCGCTAAA	55.0	50.0	58.0	58.0	2163 bp	0.5	59.5
ndhH_p2	GTGTGTAGTCTGTGAGCGAG	TTGGTACCATCCGCTAAAGC	55.0	50.0	58.0	58.0	2161 bp	0.5	59.5
ndhH_p3	GTGTGTAGTCTGTGAGCGAG	TAGCTTGGTACCATCCGCTA	55.0	50.0	58.0	57.9	2165 bp	0.5	59.2
ndhH_p4	GTGTGTAGTCTGTGAGCGAG	TCAGGTCCAATACGTTGCTG	55.0	50.0	58.0	57.9	2113 bp	0.5	59.0
ndhH_p5	GTGTGTAGTCTGTGAGCGAG	TGACTGCTATTGTTGGTCCG	55.0	50.0	58.0	57.9	2238 bp	0.5	59.0