Hotspot

ccsA

SSC · 135685-136656 · 972 bp

Back to Philodendron

Pi 0.443

MarkerSeek score 80.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 360
pi: 0.443
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TTGCATTGCCAGGAATGAGT	GTGAAATCGGTAGACACGCT	45.0	50.0	58.1	58.0	1688 bp	0.5	59.4
ccsA_p2	TTGCATTGCCAGGAATGAGT	TGAAATCGGTAGACACGCTG	45.0	50.0	58.1	58.0	1687 bp	0.5	59.3
ccsA_p3	TGACGAAATGGGAGGGGTAT	GTGAAATCGGTAGACACGCT	50.0	50.0	58.1	58.0	1762 bp	0.5	59.0
ccsA_p4	TGACGAAATGGGAGGGGTAT	TGAAATCGGTAGACACGCTG	50.0	50.0	58.1	58.0	1761 bp	0.5	59.0
ccsA_p5	CATTGCCAGGAATGAGTGGT	GTGAAATCGGTAGACACGCT	50.0	50.0	58.2	58.0	1685 bp	0.5	58.7