Hotspot

rpl32-ndhF

SSC · 137668-138945 · 1278 bp

Back to Philodendron

Pi 0.458

MarkerSeek score 82.80

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1278
variable_sites: 541
pi: 0.458
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	GTTGCGGTAAGGGTAAGTCA	CTGCAATTGGTCGTGTGAAC	50.0	50.0	57.5	58.0	2302 bp	0.5	56.0
rpl32-ndhF_p2	GTTGCGGTAAGGGTAAGTCA	GGGAGTGGAAGGAAGGGTAT	50.0	55.0	57.5	58.1	1838 bp	0.5	55.5
rpl32-ndhF_p3	GTTGCGGTAAGGGTAAGTCA	AGGAAAAGCCCACATACGAC	50.0	50.0	57.5	57.9	1917 bp	0.5	55.4
rpl32-ndhF_p4	GTTGCGGTAAGGGTAAGTCA	GAGGCACACAGAGTAGCAAA	50.0	50.0	57.5	57.8	2480 bp	0.5	55.0
rpl32-ndhF_p5	GTTGCGGTAAGGGTAAGTCA	AAAAGCCCACATACGACGAA	50.0	45.0	57.5	57.8	1914 bp	0.5	54.9