Hotspot

ndhE-psaC

SSC · 132970-133588 · 619 bp

Back to Philodendron

Pi 0.437

MarkerSeek score 82.90

Primer available yes

Label ndhE-psaC

Feature Metadata

label_name: ndhE-psaC
feature_type: igs
region: SSC
length_bp: 619
variable_sites: 249
pi: 0.437
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-psaC_p1	GTCGAAGCATGGTTAGAGCA	GAAGAGCCCGCGAATATAGG	50.0	55.0	58.0	58.0	1294 bp	0.5	59.9
ndhE-psaC_p2	GTCGAAGCATGGTTAGAGCA	GGAAGAGCCCGCGAATATAG	50.0	55.0	58.0	58.0	1295 bp	0.5	59.9
ndhE-psaC_p3	TCGAAGCATGGTTAGAGCAC	GAAGAGCCCGCGAATATAGG	50.0	55.0	58.0	58.0	1293 bp	0.5	59.9
ndhE-psaC_p4	TCGAAGCATGGTTAGAGCAC	GGAAGAGCCCGCGAATATAG	50.0	55.0	58.0	58.0	1294 bp	0.5	59.9
ndhE-psaC_p5	TCGAAGCATGGTTAGAGCAC	TACTGCGAGTTGTCTCATGC	50.0	50.0	58.0	58.0	1083 bp	0.5	59.9