Hotspot

rpl22-rps19

LSC · 93983-94080 · 98 bp

Back to Philodendron

Pi 0.133

MarkerSeek score 82.10

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 98
variable_sites: 13
pi: 0.133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	GTACGCGGTTTACTCGAGTT	GACATGCGCGAAATGACAAT	50.0	45.0	58.0	57.8	174-182 bp	1.0	84.2
rpl22-rps19_p2	TTCTGTACGCGGTTTACTCG	GACATGCGCGAAATGACAAT	50.0	45.0	58.0	57.8	178-186 bp	1.0	84.0
rpl22-rps19_p3	GTACGCGGTTTACTCGAGTT	GGAGAATTCGCACCTACTCG	50.0	55.0	58.0	58.2	205-213 bp	1.0	83.5
rpl22-rps19_p4	TTCTGTACGCGGTTTACTCG	GGAGAATTCGCACCTACTCG	50.0	55.0	58.0	58.2	209-217 bp	1.0	83.4
rpl22-rps19_p5	GTACGCGGTTTACTCGAGTT	GGGAGAATTCGCACCTACTC	50.0	55.0	58.0	57.8	206-214 bp	1.0	82.9