Hotspot

ycf1

SSC · 125057-130681 · 5625 bp

Back to Phillyrea

Pi 0.00504

MarkerSeek score 45.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5625
variable_sites: 42
pi: 0.00504
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGAGTTATTAACATGGAACCCCT	GAGGGGGTCAGCATTCAAAA	39.1	50.0	57.2	58.1	186 bp	1.0	51.2
ycf1_p2	TGAGTTATTAACATGGAACCCCT	AGAAATTGGGTAGGGAGGGG	39.1	55.0	57.2	58.4	200 bp	1.0	48.6
ycf1_p3	TGAGTTATTAACATGGAACCCCTT	GAGGGGGTCAGCATTCAAAA	37.5	50.0	57.9	58.1	186 bp	1.0	48.4
ycf1_p4	TTGAGTTATTAACATGGAACCCCT	GAGGGGGTCAGCATTCAAAA	37.5	50.0	57.9	58.1	187 bp	1.0	48.3
ycf1_p5	TGAGTTATTAACATGGAACCCC	GAGGGGGTCAGCATTCAAAA	40.9	50.0	55.8	58.1	186 bp	1.0	47.7