Hotspot

ndhF-rpl32

SSC · 114294-114767 · 474 bp

Back to Phillyrea

Pi 0.0113

MarkerSeek score 48.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 474
variable_sites: 8
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	ATGCTTTTAACGCTGTCCCA	50.0	45.0	58.0	58.1	666-668 bp	1.0	79.9
ndhF-rpl32_p2	GTTGCCGTCGGAAAAAGAAG	TGCTTTTAACGCTGTCCCAT	50.0	45.0	58.0	58.1	665-667 bp	1.0	79.9
ndhF-rpl32_p3	TGCCGTCGGAAAAAGAAGAA	ATGCTTTTAACGCTGTCCCA	45.0	45.0	58.0	58.1	664-666 bp	1.0	79.8
ndhF-rpl32_p4	TGCCGTCGGAAAAAGAAGAA	TGCTTTTAACGCTGTCCCAT	45.0	45.0	58.0	58.1	663-665 bp	1.0	79.8
ndhF-rpl32_p5	TTGCCGTCGGAAAAAGAAGA	TGCTTTTAACGCTGTCCCAT	45.0	45.0	58.0	58.1	664-666 bp	1.0	79.8