Hotspot

ycf4-cemA

LSC · 62599-63468 · 870 bp

Back to Phillyrea

Pi 0.00384

MarkerSeek score 61.60

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 870
variable_sites: 5
pi: 0.00384
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CATTCCCTTGACCCGTACTG	GAGAAATCCACCAGGGCAAA	55.0	50.0	58.0	58.1	1045-1049 bp	1.0	79.8
ycf4-cemA_p2	ATTCCCTTGACCCGTACTGA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	1044-1048 bp	1.0	79.6
ycf4-cemA_p3	TTCCCTTGACCCGTACTGAT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	1043-1047 bp	1.0	79.6
ycf4-cemA_p4	CTTGCGCGTACCAATTGAAG	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	970-974 bp	1.0	79.1
ycf4-cemA_p5	GAACAAAAAGCCGCCGAATT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.2	58.1	999-1003 bp	1.0	78.3