Hotspot

psbZ-trnG(GCC)

LSC · 37879-38170 · 292 bp

Back to Phillyrea

Pi 0.0114

MarkerSeek score 58.70

Primer available yes

Label psbZ-trnG(GCC)

Feature Metadata

label_name: psbZ-trnG(GCC)
feature_type: igs
region: LSC
length_bp: 292
variable_sites: 5
pi: 0.0114
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG(GCC)_p1	TTGCTTCTCCTGATGGTTGG	AATCGAACCCGCATCTTCTC	50.0	50.0	57.8	58.1	459-460 bp	1.0	79.1
psbZ-trnG(GCC)_p2	TTGCTTCTCCTGATGGTTGG	GAATCGAACCCGCATCTTCT	50.0	50.0	57.8	58.1	460-461 bp	1.0	79.1
psbZ-trnG(GCC)_p3	GCTTCTCCTGATGGTTGGTC	AATCGAACCCGCATCTTCTC	55.0	50.0	58.3	58.1	457-458 bp	1.0	78.7
psbZ-trnG(GCC)_p4	GCTTCTCCTGATGGTTGGTC	GAATCGAACCCGCATCTTCT	55.0	50.0	58.3	58.1	458-459 bp	1.0	78.7
psbZ-trnG(GCC)_p5	GGGTTAGTCTTTCTGGTGGG	AATCGAACCCGCATCTTCTC	55.0	50.0	57.5	58.1	394-395 bp	1.0	77.0