Hotspot

atpH-atpI

LSC · 14655-15723 · 1069 bp

Back to Phillyrea

Pi 0.00187

MarkerSeek score 59.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1069
variable_sites: 3
pi: 0.00187
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACAGAAGCGGCAGAAATCA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.1	58.3	1203 bp	1.0	77.3
atpH-atpI_p2	TACCCTCTACAGCTTGACCC	TCCCTGTCATGTTCCTTGGA	55.0	50.0	58.1	58.3	1288 bp	1.0	77.0
atpH-atpI_p3	GCGATACCCTCTACAGCTTG	TCCCTGTCATGTTCCTTGGA	55.0	50.0	57.9	58.3	1292 bp	1.0	77.0
atpH-atpI_p4	CAGCAGCAATAACAGAAGCG	TCCCTGTCATGTTCCTTGGA	50.0	50.0	57.8	58.3	1213 bp	1.0	76.8
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	TCCCTGTCATGTTCCTTGGA	50.0	50.0	57.8	58.3	1220 bp	1.0	76.4