Hotspot

trnG(GCC)-trnR(UCU)

LSC · 10858-11021 · 164 bp

Back to Phillyrea

Pi 0.0122

MarkerSeek score 60.30

Primer available yes

Label trnG(GCC)-trnR(UCU)

Feature Metadata

label_name: trnG(GCC)-trnR(UCU)
feature_type: igs
region: LSC
length_bp: 164
variable_sites: 3
pi: 0.0122
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG(GCC)-trnR(UCU)_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	247 bp	1.0	64.3
trnG(GCC)-trnR(UCU)_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	250 bp	1.0	64.1
trnG(GCC)-trnR(UCU)_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	253 bp	1.0	63.6
trnG(GCC)-trnR(UCU)_p4	AGCCTTCCAAGCTAACGATG	CATTAGACAATGGACGCTGT	50.0	45.0	58.0	55.9	228 bp	1.0	63.6
trnG(GCC)-trnR(UCU)_p5	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	256 bp	1.0	63.4