Hotspot

trnS(GCU)-trnG(GCC)

LSC · 9417-10109 · 693 bp

Back to Phillyrea

Pi 0.00866
MarkerSeek score 59.00
Primer available yes
Label trnS(GCU)-trnG(GCC)

Feature Metadata

label_name
trnS(GCU)-trnG(GCC)
feature_type
igs
region
LSC
length_bp
693
variable_sites
9
pi
0.00866
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS(GCU)-trnG(GCC)_p1 CAATCCGACGCTTTAGTCCA AGACCGAAAGACCCTTTAACT 50.0 42.9 58.0 56.6 799-800 bp 1.0 61.6
trnS(GCU)-trnG(GCC)_p2 AATCCGACGCTTTAGTCCAC AGACCGAAAGACCCTTTAACT 50.0 42.9 58.0 56.6 798-799 bp 1.0 61.6
trnS(GCU)-trnG(GCC)_p3 ATCAACGGAACCGGAAAGAG AGACCGAAAGACCCTTTAACT 50.0 42.9 57.9 56.6 863-864 bp 1.0 60.8
trnS(GCU)-trnG(GCC)_p4 CATCAACGGAACCGGAAAGA AGACCGAAAGACCCTTTAACT 50.0 42.9 58.2 56.6 864-865 bp 1.0 60.0
trnS(GCU)-trnG(GCC)_p5 TCATCAACGGAACCGGAAAG AGACCGAAAGACCCTTTAACT 50.0 42.9 58.2 56.6 865-866 bp 1.0 60.0