Hotspot

rpl14

LSC · 83101-83469 · 369 bp

Back to Philadelphus

Pi 0

MarkerSeek score 23.80

Primer available yes

Label rpl14

Feature Metadata

label_name: rpl14
feature_type: gene
region: LSC
length_bp: 369
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 23.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14_p1	ACCCTACTATAACCATATCACGC	CGCCTTTTGCATTGAACGAA	43.5	45.0	57.3	58.2	439-440 bp	1.0	49.1
rpl14_p2	ACCCTACTATAACCATATCACGC	TTCGCCTTTTGCATTGAACG	43.5	45.0	57.3	58.2	441-442 bp	1.0	49.1
rpl14_p3	CCCTACTATAACCATATCACGCT	CGCCTTTTGCATTGAACGAA	43.5	45.0	57.1	58.2	438-439 bp	1.0	47.0
rpl14_p4	CCCTACTATAACCATATCACGCT	TTCGCCTTTTGCATTGAACG	43.5	45.0	57.1	58.2	440-441 bp	1.0	47.0
rpl14_p5	CCCTACTATAACCATATCACGCTC	CGCCTTTTGCATTGAACGAA	45.8	45.0	58.1	58.2	438-439 bp	1.0	45.4