Hotspot

rpl32-trnL-UAG

SSC · 116046-116906 · 861 bp

Back to Philadelphus

Pi 0.00349

MarkerSeek score 60.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 861
variable_sites: 3
pi: 0.00349
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGCGCTAAAGGCCTTTTCAT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.2	58.0	971-972 bp	1.0	79.0
rpl32-trnL-UAG_p2	AGCGCTAAAGGCCTTTTCAT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.2	58.0	972-973 bp	1.0	79.0
rpl32-trnL-UAG_p3	AGCGCTAAAGGCCTTTTCAT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.2	57.9	981-982 bp	1.0	78.0
rpl32-trnL-UAG_p4	AGCGCTAAAGGCCTTTTCAT	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	58.2	57.8	980-981 bp	1.0	77.5
rpl32-trnL-UAG_p5	AGCGCTAAAGGCCTTTTCAT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	58.2	57.8	979-980 bp	1.0	77.5