Hotspot

rpl14-rpl16

LSC · 83470-83608 · 139 bp

Back to Philadelphus

Pi 0.0146

MarkerSeek score 62.30

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 139
variable_sites: 2
pi: 0.0146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	AGCATATCGGCGATTGCTAG	AGCAGCGTCTAAAATGCCTA	50.0	45.0	58.0	57.3	285-287 bp	1.0	75.6
rpl14-rpl16_p2	AGCATATCGGCGATTGCTAG	TAGCAGCGTCTAAAATGCCT	50.0	45.0	58.0	57.3	286-288 bp	1.0	75.6
rpl14-rpl16_p3	CATATCGGCGATTGCTAGCT	AGCAGCGTCTAAAATGCCTA	50.0	45.0	58.0	57.3	283-285 bp	1.0	75.6
rpl14-rpl16_p4	CATATCGGCGATTGCTAGCT	TAGCAGCGTCTAAAATGCCT	50.0	45.0	58.0	57.3	284-286 bp	1.0	75.6
rpl14-rpl16_p5	GAGCATATCGGCGATTGCTA	AGCAGCGTCTAAAATGCCTA	50.0	45.0	58.0	57.3	286-288 bp	1.0	75.6