Hotspot

rps18-rpl20

LSC · 70728-71029 · 302 bp

Back to Philadelphus

Pi 0.00662

MarkerSeek score 42.20

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 302
variable_sites: 2
pi: 0.00662
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	AATCGACACAATACGCCGAA	TAAGGGGCTCCGGTGTATAG	45.0	55.0	58.0	58.0	1567 bp	1.0	80.2
rps18-rpl20_p2	AATCGACACAATACGCCGAA	CGTTTGTGGATCACTCGGAT	45.0	50.0	58.0	58.0	1070 bp	1.0	80.2
rps18-rpl20_p3	GGAAGCAAGGAAGGGGAAAA	TAAGGGGCTCCGGTGTATAG	50.0	55.0	58.0	58.0	1444 bp	1.0	80.2
rps18-rpl20_p4	GGAAGCAAGGAAGGGGAAAA	CGTTTGTGGATCACTCGGAT	50.0	50.0	58.0	58.0	947 bp	1.0	80.2
rps18-rpl20_p5	AATCGACACAATACGCCGAA	GGTTATCGTAGCCAAAGCCA	45.0	50.0	58.0	58.0	1385 bp	1.0	80.0