Hotspot

atpB-rbcL

LSC · 56297-57045 · 749 bp

Back to Philadelphus

Pi 0.00268

MarkerSeek score 60.70

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 749
variable_sites: 2
pi: 0.00268
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTCAAGCGTGGAAATCCCA	AACACCCGCTTTGAATCCAA	45.0	45.0	58.0	58.3	847-849 bp	1.0	78.0
atpB-rbcL_p2	TGGAAATCCCAGGACCAGAA	AACACCCGCTTTGAATCCAA	50.0	45.0	58.2	58.3	838-840 bp	1.0	76.1
atpB-rbcL_p3	TTTCAAGCGTGGAAATCCCA	TAACACCCGCTTTGAATCCA	45.0	45.0	58.0	57.4	848-850 bp	1.0	75.8
atpB-rbcL_p4	GGAAATCCCAGGACCAGAAG	AACACCCGCTTTGAATCCAA	55.0	45.0	57.6	58.3	837-839 bp	1.0	74.7
atpB-rbcL_p5	TTTCAAGCGTGGAAATCCCA	CCCGCTTTGAATCCAACACT	45.0	50.0	58.0	58.8	843-845 bp	1.0	74.4