Hotspot

rps4-trnT-UGU

LSC · 47578-47972 · 395 bp

Back to Philadelphus

Pi 0.00266
MarkerSeek score 60.70
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
395
variable_sites
1
pi
0.00266
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 ACGCGATTGGTTTCTAAGCT CTCAGAGGTTAGAGCATCGC 45.0 55.0 57.9 57.9 550-569 bp 1.0 81.0
rps4-trnT-UGU_p2 ACGCGATTGGTTTCTAAGCT GATGGTCATCGGTTCGACTC 45.0 55.0 57.9 58.2 519-538 bp 1.0 81.0
rps4-trnT-UGU_p3 ACGCGATTGGTTTCTAAGCT CTGTTATGTGAGCCCGCTTA 45.0 50.0 57.9 57.7 571-590 bp 1.0 79.5
rps4-trnT-UGU_p4 ACGCGATTGGTTTCTAAGCT TGTTATGTGAGCCCGCTTAG 45.0 50.0 57.9 57.7 570-589 bp 1.0 79.5
rps4-trnT-UGU_p5 ACGCGATTGGTTTCTAAGCT CCGCTTAGCTCAGAGGTTAG 45.0 55.0 57.9 57.5 558-577 bp 1.0 78.1