Hotspot

trnC-GCA-petN

LSC · 28939-29772 · 834 bp

Back to Philadelphus

Pi 0.00719

MarkerSeek score 62.90

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 834
variable_sites: 6
pi: 0.00719
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTTGTATCGTTTTGGCGGC	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.9	58.2	956 bp	1.0	78.9
trnC-GCA-petN_p2	GGGGACTGCAAATCCTTTCA	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	917 bp	1.0	78.7
trnC-GCA-petN_p3	TTCACCCCAGTTCAAATCCG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	901 bp	1.0	78.6
trnC-GCA-petN_p4	TTTTGTATCGTTTTGGCGGC	CTACCATTAACGCAGCCCAA	45.0	50.0	57.9	58.3	969 bp	1.0	78.0
trnC-GCA-petN_p5	TTTTGTATCGTTTTGGCGGC	TACCATTAACGCAGCCCAAG	45.0	50.0	57.9	58.3	968 bp	1.0	78.0