Hotspot

atpH-atpI

LSC · 14427-15551 · 1125 bp

Back to Philadelphus

Pi 0.00267

MarkerSeek score 60.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1125
variable_sites: 3
pi: 0.00267
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GGCAGAAACCAGTGGATTCA	CAAGCGGTATCCAAGCTCTT	50.0	50.0	58.1	58.0	1223 bp	1.0	79.3
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	CAAGCGGTATCCAAGCTCTT	50.0	50.0	57.9	58.0	1290 bp	1.0	79.1
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	CAAGCGGTATCCAAGCTCTT	45.0	50.0	58.2	58.0	1235 bp	1.0	78.5
atpH-atpI_p4	GCAGTACCTTGACCAACTCC	CAAGCGGTATCCAAGCTCTT	55.0	50.0	58.2	58.0	1294 bp	1.0	78.5
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	CAAGCGGTATCCAAGCTCTT	50.0	50.0	57.8	58.0	1249 bp	1.0	78.3