Hotspot

trnS-GCU-trnG-UCC

LSC · 9054-9862 · 809 bp

Back to Philadelphus

Pi 0

MarkerSeek score 60.40

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 809
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	AATCCAAACCGAAAGACCCC	50.0	50.0	58.0	58.1	903-922 bp	1.0	81.0
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	AATCCAAACCGAAAGACCCC	50.0	50.0	58.0	58.1	902-921 bp	1.0	81.0
trnS-GCU-trnG-UCC_p3	CCATCAACGGAAAGAGAGGG	AATCCAAACCGAAAGACCCC	55.0	50.0	58.0	58.1	963-982 bp	1.0	80.7
trnS-GCU-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	AATCCAAACCGAAAGACCCC	50.0	50.0	58.2	58.1	957-976 bp	1.0	79.7
trnS-GCU-trnG-UCC_p5	AGGAAGCCATCAACGGAAAG	AATCCAAACCGAAAGACCCC	50.0	50.0	58.2	58.1	969-988 bp	1.0	79.5