Hotspot

ycf1

SSC · 127054-132522 · 5469 bp

Back to Phellodendron

Pi 0.000183

MarkerSeek score 52.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5469
variable_sites: 1
pi: 0.000183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGCCCTATTTCGCCATTCTT	ACAAATTGTTACAAGCCCCA	50.0	40.0	58.3	55.7	260 bp	1.0	59.6
ycf1_p2	CCGCTTAACCTGTTTTTCCG	ACAAATTGTTACAAGCCCCA	50.0	40.0	57.7	55.7	190 bp	1.0	59.6
ycf1_p3	GGCCCTATTTCGCCATTCTT	GTCTTTTTCACAATCCCAAGCA	50.0	40.9	58.3	57.7	292 bp	1.0	59.2
ycf1_p4	CCGCTTAACCTGTTTTTCCG	GTCTTTTTCACAATCCCAAGCA	50.0	40.9	57.7	57.7	222 bp	1.0	59.2
ycf1_p5	GTTGATTGGCCCTATTTCGC	ACAAATTGTTACAAGCCCCA	50.0	40.0	57.5	55.7	267 bp	1.0	58.2