Hotspot

rpl32

SSC · 116300-116473 · 174 bp

Back to Phellodendron

Pi 0.00575

MarkerSeek score 55.80

Primer available yes

Label rpl32

Feature Metadata

label_name: rpl32
feature_type: gene
region: SSC
length_bp: 174
variable_sites: 1
pi: 0.00575
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32_p1	GTCTTTCACATCCAATTTGAGCA	TTTGAGTCAGGCGGATTCAG	39.1	50.0	57.9	57.9	241 bp	1.0	54.5
rpl32_p2	TGTCTTTCACATCCAATTTGAGC	TTTGAGTCAGGCGGATTCAG	39.1	50.0	57.9	57.9	242 bp	1.0	54.5
rpl32_p3	TCACATCCAATTTGAGCAATGA	TTTGAGTCAGGCGGATTCAG	36.4	50.0	56.6	57.9	236 bp	1.0	52.3
rpl32_p4	TCTTTCACATCCAATTTGAGCA	TTTGAGTCAGGCGGATTCAG	36.4	50.0	56.5	57.9	240 bp	1.0	51.8
rpl32_p5	GTCTTTCACATCCAATTTGAGCA	TTTTGAGTCAGGCGGATTCA	39.1	45.0	57.9	57.4	242 bp	1.0	50.9