Hotspot

ndhF-rpl32

SSC · 115397-116299 · 903 bp

Back to Phellodendron

Pi 0

MarkerSeek score 35.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 903
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCGAAGATGTTTTGTTGCGA	CTGCCCAATACCCCCTTTTT	45.0	50.0	58.0	58.0	1096 bp	1.0	79.4
ndhF-rpl32_p2	GCGAAGATGTTTTGTTGCGA	AACGAAAAAGCCTTCAACGC	45.0	45.0	58.0	58.2	1115 bp	1.0	78.4
ndhF-rpl32_p3	CCACATACGGCGAAGATGTT	CTGCCCAATACCCCCTTTTT	50.0	50.0	58.3	58.0	1105 bp	1.0	76.8
ndhF-rpl32_p4	ACGGCGAAGATGTTTTGTTG	CTGCCCAATACCCCCTTTTT	45.0	50.0	57.6	58.0	1099 bp	1.0	76.4
ndhF-rpl32_p5	AAAGTAGAAGTCCCACCCCT	CTGCCCAATACCCCCTTTTT	50.0	50.0	57.6	58.0	1070 bp	1.0	76.1