Hotspot

psbZ-trnG-GCC

LSC · 38468-39018 · 551 bp

Back to Phellodendron

Pi 0.0449

MarkerSeek score 86.40

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 551
variable_sites: 24
pi: 0.0449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 86.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CGGATGGTTGGTCGAGTAAC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.4	57.8	699-731 bp	1.0	80.3
psbZ-trnG-GCC_p2	TTTGCTTCTCCGGATGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.5	57.8	709-741 bp	1.0	79.4
psbZ-trnG-GCC_p3	ATTTGCTTCTCCGGATGGTT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.5	57.8	710-742 bp	1.0	79.0
psbZ-trnG-GCC_p4	CGGATGGTTGGTCGAGTAAC	ACATTACTTTACCCTGGCGG	55.0	50.0	58.4	57.6	738-770 bp	1.0	78.1
psbZ-trnG-GCC_p5	TTTGCTTCTCCGGATGGTTG	ACATTACTTTACCCTGGCGG	50.0	50.0	58.5	57.6	748-780 bp	1.0	77.3