Hotspot

rps16

Genome · 3613-4702 · 1090 bp

Back to Phegopteris

Pi 0.0741

MarkerSeek score 79.60

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: Genome
length_bp: 1090
variable_sites: 78
pi: 0.0741
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	CGCTAAAGTGCTGAGCTACA	GGGGCATGATACCAAAGGAG	50.0	55.0	58.0	58.0	1826-1849 bp	1.0	87.3
rps16_p2	CTTGAACTACCTGCGTCTCC	GGGGCATGATACCAAAGGAG	55.0	55.0	58.0	58.0	1672-1695 bp	1.0	87.1
rps16_p3	CGCTAAAGTGCTGAGCTACA	TGGGCATGGAAGATCATTCG	50.0	50.0	58.0	58.0	1860-1883 bp	1.0	87.1
rps16_p4	CTTGAACTACCTGCGTCTCC	TGGGCATGGAAGATCATTCG	55.0	50.0	58.0	58.0	1706-1729 bp	1.0	86.9
rps16_p5	CTGTAAGCTGGGCCCATTAG	GGGGCATGATACCAAAGGAG	55.0	55.0	58.0	58.0	1490 bp	0.5	59.7